If you had an oil spill in a driveway, for example, you could pour a little Tide and water on it and then come out an hour later and rinse everything away, including the oil. But these days, things are different. Because once we realized the health hazard these chemicals posed to our water supply, some things had to change. What is phosphorus and how does it affect water systems?
View previous table Soils contaminated in the field were collected and comparatively studied for the effect of microbes and nutrients on the overall degradation of diesel TPH over a 12week study period by Bento et al The research design was done to compare the process of combination therapy with bioaugmentation.
The researchers studied the degradation of diesel oil in the light CC23 and heavy CC40 range. Notably, the authors stated that the addition of nutrients did not significantly impact the number of diesel degrading microorganisms and heterotrophic population and acknowledged a major limitation in a lack of detailed site specification and characterization.
This they claim was very necessary prior to deciding the technique to be adopted for bioremediation. Conclusively, the researchers advocated that nutrient provision for remediation was more a less not as important as inoculation of proven microbial strains with biodegradative qualities; although the difference in these rates seemed not to be statistically significant.
Employing a combination therapy is also Uses of bioaugmentation in TPH degradation with spent motor oil being the contaminant Abdusalam and Omale, In a study that lasted 42 days, biostimulation had the highest TPH removal rate of Other categories water amendment and control had Inoculation with a consortium of Bacillus Sp, Pseudomonas Sp, and Proteus Sp all known degraders and NPK fertilizer did not improve the degradation rates as only nutrient amended NPK recorded a better overall percentage reduction.
Using a combination of experimental techniques, Alvarez et al studied bioremediation of tropical soil microcosms by bioenrichment, bioaugmentation and natural attenuation. The authors studied the degradation rate of n-alkanes as well as TPH.
Crude oil contaminated soil was augmented with actinomycetes strain, combination with sodium chloride and a final category monitored closely. Their results indicated that degradation of n-alkanes was uniform in all categories nullifying a dependence on amendment or augmentation since the monitored category showed degradation as well.
Interestingly, they reported that total bacterial community in the soils was mainly affected by the experimental period of time and concluded that monitored attenuation was the best strategy for remediation with or without addition of sodium chloride. Eun-Hee et al employed a combination of Rhodococcus Sp.
EH and a surfactant for the bioremediation of diesel contaminated soils and compared this treatment with bioaugmentation using Rhodococcus species only.
As observed by the authors, the addition of the surfactant had no significant effect on the remediation performance. Even though surfactants alone achieved significant remediation rates as reported by Reznik et altheir presence in the research under focus affected neither TPH removability nor the physiological properties of the microorganisms.
A study demonstrated the feasibility of using microorganisms and nutrients for bioremediation Abdulsalam et al They conclusively indicated that a combination technique could be used to develop a realistic treatment technology for soils contaminated with spent motor oil.
Recent Strategies for Bioremediation The use of certain genetically engineered microorganisms to influence their ability to utilize specific contaminants such as hydrocarbons and pesticides is gaining grounds. This technique had an early mention in the late s and early s.JRW On the Go..
Look for JRW at the following conferences in the near future. If we are in your area for one of these meetings and you would like a JRW representative to conduct . Bioaugmentation has been proven successful in cleaning up of sites contaminated with aromatic compounds but still faces many environmental problems.
One of the most difficult issues is survival of strains introduced to soil.
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Successful bioaugmentation treatments depend on the use of inocula consisting of microbial strains or microbial consortia that have been well adapted to the site to be decontaminated. Foreign microorganisms (those in inocula) have been applied successfully but their efficiency depends on ability to compete with indigenous microorganisms.
Environmental Engineering and Consulting Company that provides onsite training, consulting, Bioaugmentation products, Lab services, wastewater Training materials, Wastewater Training and waste water eLearning training. To support successful bioaugmentation strategies, along with enhancing biodegradation kinetics of the target pollutant, the use of molecular techniques is required (monitoring both the survival and/or activity of the added microorganisms).